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Samples (30 g protein) were resolved by 420% SDS-PAGE (Biofuraw Precast Gel, Tanon Science, Shanghai, China), transferred to polyvinylidene difluoride membranes, and incubated with one of the following antibodies: LC3 (88588s, Cell Signaling Technology, 1:1000), GPX4 (ab125066, Abcam, 1:1000), p16 (1003-1-AP, Proteintech, 1:1000)

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The addition of glycine into their nutrition medium was effective, and the influencing concentration was 1 g/L, allowing for riboflavin yields of 144.7 and 184.2 mg/L, respectively ( B

The glutathione system includes glutathione, glutathione reductase, glutathione peroxidases and glutathione ''S''-transferases